Locals fear reopening the Bird in Hand goldmine in the Adelaide Hills will harm the region's reputation.
Terramin's lodged an application with the State Government to revive the site, which hasn't been mined since the 1880s.
Residents packed a meeting at Woodside on Monday 19 August to discuss the potential impact the project would have on the local wine, horticulture and tourism industries.
Jared Stringer from Bird in Hand Winery said he fears it would harm the region's reputation.
"We're not talking about a remote part of South Australia here, we're talking about the Adelaide Hills, a clean green foodbowl... a region that's producing elite wine and food on a global stage," Mr Stringer said.
Terramin CEO Richard Taylor said there's no reason why they cannot all co-exist.
"This is not a choice between tourism, wineries or mining. It's a complimentary approach that can be taken. We can work together; it's been shown at McLaren Vale, Barossa, other places where there are large quarries that coexist with winery and tourism development," Mr Taylor said.
Terramin’s 6000-page submission can be accessed here.
The State Government's guidelines for public consultation and submissions can be accessed here.
Image of a gold mine site by Khushie singh, Wikimedia